Inside Life Science
New Uses for Old Drugs
Posted August 17, 2011
Researchers have predicted new uses for drugs already on the market, thanks to computer power and public databases of genomic information. The work, described in a study published today, was led by Atul Butte, a bioinformatics researcher at Stanford University supported by the National Institutes of Health. His team uncovered promising drug treatments for 53 human diseases ranging from cancers to Crohn's disease and cardiovascular conditions.
"Many other uses of drugs remain to be discovered," says Butte, "and computational methods applied to public molecular data can help with finding these new uses."
Developing a new drug and bringing it to market can take 15 years and cost over $1 billion. Identifying ways to put FDA-approved drugs to new uses, called drug repositioning, lets researchers sidestep another long and costly path through testing. It also means that people who need drug therapies have less time to wait for them.
Butte and his team started by digging through computerized public databases to see how 100 diseases alter the activity of thousands of genes. For example, when compared to healthy cells, a disease might increase the activity of genes A, B and C and decrease the activity of genes D, E and F. They called this pattern of activity a genetic "signature."
The researchers took a similar approach to 164 different drugs, characterizing each with a genetic "signature" based on activity patterns in human cell samples that had been treated with the drug.
Finally, the team created a computer program to compare the drug and disease signatures. "We developed a computational method to match up molecular data on drugs and diseases, so that when statistically paired up, we can infer that a drug might work against a disease," Butte explains.
If a drug signature and a disease signature showed exactly the same pattern of genetic activity, the computer gave the pair a similarity score of +1. If their signatures were completely opposite, the pair received a score of -1.
Because an effective drug theoretically reverses the activity in a diseased cell, opposing signatures—scores closer to -1—indicated a better potential match for treatment.
The end result was a ranked list of potential therapeutics, where 53 of the diseases were significantly matched to drug candidates. Many of the matches confirmed relationships that were already known. For example, the steroid prednisolone is commonly given to treat inflammatory bowel disease; the two had opposing scores in Butte's analysis, making them a good therapeutic match.
But the study also turned up some surprising results. For instance, topiramate, an anticonvulsant used to treat epilepsy, emerged as a better match for inflammatory bowel disease than prednisolone. Another surprising connection appeared between cimetidine, an anti-ulcer drug, and the lung cancer adenocarcinoma.
To put their findings to the test, Butte's team conducted experiments using cimetidine to treat adenocarcinoma and topiramate to treat inflammatory bowel disease.
"We show that these two drugs actually do show signs of efficacy when tested on rat and mouse models for these two diseases," Butte explains.
In the lab, the researchers found that human lung cancer cells treated with cimetidine in Petri dishes grew slower than untreated cells. In mouse models, increasing doses of the inexpensive anti-ulcer drug also slowed tumor growth.
When Butte and colleagues tested topiramate in rat models of inflammatory bowel disease, they found that the drug reduced swelling and damage to colon tissue—sometimes more than prednisolone.
Even though more studies are needed to see if the same trends are true in humans, Butte's outside-the-box approach to drug discovery could potentially be applied to treat a range of diseases in unanticipated ways. It also highlights the value of computational analysis and public databases to learn more about how diseases and drugs work at the molecular level.
"This work is still at an early stage," says Rochelle Long of the National Institutes of Health, which partly funded the research. "But it is a promising proof of principle for a creative, fast and affordable approach to discovering new uses for drugs we already have in our therapeutic arsenal."
- Dr. Data: Profile of Atul Butte
- NIH Pharmacogenomics Research Network
- Listen to a radio interview with Atul Butte about his research
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